HUman Lung model for Key screens and preclinical validation of antivirals against SARS-CoV-2 infection

Abstract: "Rapid screens of potential antivirals are urgently needed to mitigate the current COVID-19 outbreaks. Ideally, such screens should be deployed in models that closely reflect the biologically and clinically relevant environment of the virus infection, because this may accelerate the discovery of promising drug candidates. We propose to develop an in vitro model of human lung infection with SARS-CoV-2 infection for initial screening and in depth validation of hits. Our laboratories possess strong virological expertise, including direct work with SARS-CoV-2. We have established cultures of human respiratory epithelial cells in monolayers or in 3D models of culture at air/liquid interfaces. The SME partner has established a technology for the development of immortalized cell lines and has been expanding stocks of cells from upper and lower parts of the respiratory system. We propose to combine these models to develop (1) direct screens of antiviral compounds in 2D cultures of human respiratory cells and (2) validation assays in 3D models."; Research Type: discovery; Study population: not applicable