Rapid testing for CoV-2 RNA in swabs

  • Funded by Bundesministerium für Bildung und Forschung [German Federal Ministry of Education and Research] (BMBF)
  • Total publications:1 publications

Grant number: 01KI20184

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Key facts

  • Disease

    COVID-19
  • Start & end year

    2020
    2021
  • Known Financial Commitments (USD)

    $471,635.77
  • Funder

    Bundesministerium für Bildung und Forschung [German Federal Ministry of Education and Research] (BMBF)
  • Principle Investigator

    Pending
  • Research Location

    Germany, Europe
  • Lead Research Institution

    Uniklinik Köln
  • Research Category

    Pathogen: natural history, transmission and diagnostics

  • Research Subcategory

    Diagnostics

  • Special Interest Tags

    Gender

  • Study Subject

    Clinical

  • Clinical Trial Details

    Not applicable

  • Broad Policy Alignment

    Pending

  • Age Group

    Unspecified

  • Vulnerable Population

    Unspecified

  • Occupations of Interest

    Unspecified

Abstract

clinical trial - Rapid and extensive testing of both personnel and patients is crucial for proper management SARS-CoV-2 infections and decision-making in times of the current pandemic outbreak. However, point-of-care (POC) testing in emergency units or outpatient clinics is not possible yet at a larger scale using established PCR-based methods. Therefore, we tested two PCR-independent methods to detect viral RNA from primary material (swabs) circumventing the need of RNA preparation. Firstly, SHERLOCK, developed by Feng Zhang at MIT(1), uses Recombinase Polymerase Amplification (RPA) combined with Cas13a-dependent detection of RNA as a second step followed by a lateral-flow assay on a test strip. As a simple alternative, loop-mediated amplification (LAMP) only requires one incubation step with detection based either on a colorimetric assay or lateral flow strips. SHERLOCK and LAMP can be performed in 60 or 30 minutes, respectively. Based on our preliminary data, we can show that both methods work on isolated viral RNA as well as on direct material from pharyngeal swabs - a crucial pre-requisite for point-of-care testing. However, both approaches still lack sensitivity as compared to standard qPCR testing. In this proposal we aim to (1) improve and combine aspects of both SHERLOCK and LAMP to develop an easy and fast diagnostic method allowing for viral RNA detection without RNA isolation at a sensitivity comparable to qPCR and (2) to test these approaches in the setting of an emergency room.

Publicationslinked via Europe PMC

Last Updated:39 minutes ago

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Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay.