Clinical evaluation of SARS-CoV-2 subunit vaccine in a Phase I human clinical study

AbstractThe novel SARS-CoV-2 is the cause of the coronavirus (CoV) disease (COVID-19) outbreak that currently posea serious pandemic threat to public health. A safe vaccine that rapidly induces long-lasting virus-specific immuneresponses is urgently needed. The CoV spike (S) protein, a characteristic structural component of the viralenvelope, is considered a key target for vaccines against CoV infection, as we and others have previouslydemonstrated for severe acute respiratory syndrome (SARS) and middle east respiratory syndrome (MERS)CoV infections. The safety profile of non-infectious recombinant protein subunit vaccines makes them suitablefor SARS-CoV-2 vaccine candidates for preclinical testing. To develop a SARS-CoV-2 vaccine, we constructedSARS-CoV-2-S1 subunit constructs and established an intracutaneous delivery platform using a novel,dissolving microneedle array (MNA) that enhances the immunogenicity of these subunit vaccines in mice, asdetermined by S1 specific viral titers in serum. Here, we propose to evaluate this PittCoVacc vaccine in a phaseI clinical trial through a single specific aim designed to complete ongoing IND enabling studies, any additionalparallel studies recommended by the FDA, and then to conduct a Phase 1 clinical trial in healthy volunteers.