Point-of-Care Sensor Device for Early Stage Detection of COVID-19 Using Isothermal DNA Amplification [Funder: Carleton University COVID-19 Rapid Research Response Grants]

Grant number: unknown

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Key facts

  • Disease

    COVID-19
  • Funder

    Other Funders (Canada)
  • Principal Investigator

    Ravi Prakash
  • Research Location

    Canada
  • Lead Research Institution

    Carleton University
  • Research Priority Alignment

    N/A
  • Research Category

    Pathogen: natural history, transmission and diagnostics

  • Research Subcategory

    Diagnostics

  • Special Interest Tags

    N/A

  • Study Type

    Non-Clinical

  • Clinical Trial Details

    N/A

  • Broad Policy Alignment

    Pending

  • Age Group

    Not Applicable

  • Vulnerable Population

    Not applicable

  • Occupations of Interest

    Not applicable

Abstract

The COVID-19 pandemic has caused a global healthcare crisis which necessitates extensive, rigorous, and early stage screening of the virus. A significant challenge in monitoring community spread is that over 50% of carriers can be asymptomatic and still spread the infection. As a result, extensive testing at centralized laboratories would be challenging. Existing DNA and antibody biomarker-based COVID-19 tests have also been limited due to their complexity and dependence on benchtop instruments. There is a strong push towards designing rapid point-of-care (POC) nucleic acid test (NAT) systems to extract and amplify the viral RNA using improved DNA molecular probes to facilitate miniaturization and reduce screening time. Our interdisciplinary research team possesses expertise in POC NAT system design for influenza and arboviruses, and novel DNA biomarker discovery. We have initiated a sustainable partnership to significantly improve the existing sensor device design and incorporate novel isothermal DNA amplification primers for COVID-19 testing.