Global genome screening with CRISPRko libraries to identify essential factors in SARS-COV2 infection and replication

  • Funded by Fundação de Amparo à Pesquisa do Estado de São Paulo [São Paulo Research Foundation] (FAPESP)
  • Total publications:3 publications

Grant number: 2020/04860-8

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Key facts

  • Disease

    COVID-19
  • Start & end year

    2020
    2022
  • Known Financial Commitments (USD)

    $71,176.44
  • Funder

    Fundação de Amparo à Pesquisa do Estado de São Paulo [São Paulo Research Foundation] (FAPESP)
  • Principle Investigator

    Pending
  • Research Location

    Brazil, Americas
  • Lead Research Institution

    Universidade de São Paulo
  • Research Category

    Pathogen: natural history, transmission and diagnostics

  • Research Subcategory

    Pathogen morphology, shedding & natural history

  • Special Interest Tags

    Gender

  • Study Subject

    Non-Clinical

  • Clinical Trial Details

    N/A

  • Broad Policy Alignment

    Pending

  • Age Group

    Not Applicable

  • Vulnerable Population

    Not applicable

  • Occupations of Interest

    Not applicable

Abstract

The present decade begins with a worldwide health emergency, the new coronavirus pandemic, the cause of severe acute respiratory syndrome 2 (COVID-19), also known as SARS-Cov2. To date, there are more than 900,000 positive cases that have resulted in more than 44,000 deaths in 180 countries. In view of these epidemiological data, strategies are needed to contain the transmission of the virus and mainly effective alternatives in the treatment of this disease. In this context, with the project we aim to assess the versatility of using a CRISPRko library to identify critical factors for SARS-Cov2 infection and replication. This library consists of a group of 77,441 guides directed to approximately 19 thousand human genes. This tool will allow us to globally screen the human genome, through CRISPR / Cas9-mediated gene deletion in human lung epithelial cells. After selection against essential genes for cell viability, we submitted the cells to five rounds of lethal infection with human isolate (from Brazil) of a SARS-Cov2 strain. Finally, we will be able to determine genes and pathways involved in viral replication and infection, by sequencing the surviving cells and detecting the guides present in that population. With this high coverage strategy, we believe that we will be able to point out new potential targets for the development of therapies for this disease.

Publicationslinked via Europe PMC

Last Updated:37 minutes ago

View all publications at Europe PMC

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