CORONA - Travel-Dx - Quick test for mobility; subproject 2: Detection

The detection of SARS-Cov-2 without the need for equipment has the advantage of being able to realize a rapid on-site detection. For this purpose, LAMP products must be made visible; this is usually done by means of fluorescence and is thus linked to a readout device. The aim of this subproject is to make a readout device dispensable and to offer an immediately visible detection by means of a lateral flow assay (test strip). The task is to develop stable molecular mechanisms that act like a switch and generate an unambiguous (color) signal triggered by a positive binding process, in this case viral RNA. The scientific goal is to increase the sensitivity sufficiently without loss of specificity. The concrete goal of this project is to identify the amplification products of a LAMP reaction unambiguously in a short time, while the detection reaction itself should be completed in a few minutes.