Development of colorimetric kits for porcine circovirus 2 (PCV-2) and SARS-CoV-2 quantification using gold nanoparticles

Until the morning of March 31, 2020, 719,700 confirmed cases of SARS-Cov-2 were registered worldwide, causing a collapse in the health system of countries such as Italy, Iran and Spain. Brazil to date has recorded 4,661 confirmed cases, with 165 deaths. The transmission of the virus occurs mainly by contact with secretions and infected people, causing fever, cough, dyspnoea, myalgia or fatigue, sputum production, headache, hemoptysis and diarrhea. The diagnosis occurs after medical evaluation and laboratory tests such as RT-qPCR. However, these methodologies present as limitations a high cost for analysis of the samples, in addition to requiring specialized professionals and specific equipment. That's why, it is necessary to develop a new reliable and low cost methodology for a quick and simple diagnosis. Biosensors have stood out in the technological area as an analytical tool that can identify the presence of pathogens (antigens or nucleic acid) using nanoparticles as a base. Therefore, the objective of the present project is to develop and standardize colorimetric kits for the identification of both SARS-CoV-2 and viral RNA, using gold nanoparticles modified with the deposition of antibodies or specific probes, which, when attached to their respective target, will provide an immediate color change. This is a new technique that will promote a diagnosis in a quick, simple and low cost, in comparison with the usual methodologies. (AU) Biosensors have stood out in the technological area as an analytical tool that can identify the presence of pathogens (antigens or nucleic acid) using nanoparticles as a base. Therefore, the objective of the present project is to develop and standardize colorimetric kits for the identification of both SARS-CoV-2 and viral RNA, using gold nanoparticles modified with the deposition of antibodies or specific probes, which, when attached to their respective target, will provide an immediate color change. This is a new technique that will promote a diagnosis in a quick, simple and low cost, in comparison with the usual methodologies. (AU) Biosensors have stood out in the technological area as an analytical tool that can identify the presence of pathogens (antigens or nucleic acid) using nanoparticles as a base. Therefore, the objective of the present project is to develop and standardize colorimetric kits for the identification of both SARS-CoV-2 and viral RNA, using gold nanoparticles modified with the deposition of antibodies or specific probes, which, when attached to their respective target, will provide an immediate color change. This is a new technique that will promote a diagnosis in a quick, simple and low cost, in comparison with the usual methodologies. (AU) using gold nanoparticles modified with the deposition of antibodies or specific probes, which, when bound to their respective target, will provide an immediate change in color. This is a new technique that will promote a diagnosis in a quick, simple and low cost, in comparison with the usual methodologies. (AU) using gold nanoparticles modified with the deposition of antibodies or specific probes, which, when bound to their respective target, will provide an immediate change in color. This is a new technique that will promote a diagnosis in a quick, simple and low cost, in comparison with the usual methodologies. (AU)