SARS-Cov-2 sensor for rapid sensitive virus detection

Purpose and goal The purpose of this project is to develop Covid-19 virus receptors and sensors that combine the following features: fast response, high sensitivity and specificity, low cost (no expensive reagents) and robustness. The solution is based on rSAMs (reversible self-assembled monolayers), a biomimetic approach that can be quickly adapted to new epidemic threats and builds on ongoing collaborations between Malmö University (MaU), Linköping University (LiU) and the companies Surecapture Technologies and ArgusEye. Expected results and effects This project will result in entirely new virus diagnostic and detection technologies featuring considerably improved performance and cost benefits with respect to currently used technologies. For example, the rSAM/nanoplasmonic solution will be used for unprecedented rapid and sensitive virus detection in real time, a key need in environmental epidemiology. Moreover, rSAM-nanoplasmonics is an antibody-free detection solution with no need for labile expensive reagents. Planned approach and implementation The project aims to combine biomimetic membranes prepared by the rSAM technology of MaU/Surecapture with nanoplasmonic sensors developed at LiU and ArgusEye. To adapt rSAMs to SARS-CoV-2 detection we will introduce mobile receptor epitopes on anchoring surfaces. The resulting rSAMs will be tested for their affinity towards the SARS-CoV-2 spike protein and virus-like particles. The dynamic receptors will then be combined with LSPR sensors that enables highly sensitive label free analyte detection. The sensors will be validated against currently used RT-qPCR virus tests.