Human germinal center organoids as a tool to understand B cell biology and for the identification of monoclonal antibodies with therapeutic potential against SARS-CoV2

Within our body, B cells, a type of white blood cells, produce and release antibodies to protect us against invaders such as the coronavirus that causes the current COVID-19 pandemic. To obtain antibodies in vitro, we propose to develop a three-dimensional cell culture that replicates the function of the anatomical structures called germinal centers, where B cells activate, expand, and form antibody-secreting cells. Our aims are: (i) to develop methods for obtaining antibodies with high affinity against SARS-CoV2, using microscopic particles that will act as the "invaders" and which will transport substances to specific B cells for their activation, and (ii) to find the best culture conditions to mimic the germinal center structure and function. The proposed model will advance our understanding of B cell activation, and will collaborate with an alternative option for the prevention and treatment of COVID-19. Moreover, this model could be used to generate antibodies against other targets of interest.