Nanomaterials based-Genosensor (Nano-GS) for improved detection method of SARS-CoV-2 RNA as rRapid COVID-19 diagnosis strategy

Our research plan is to develop a biosensor, made of a special matrix that holds a G-quadruplex DNA probe, targeting specific a site of the SARS-CoV-2 viral RNA. Binding interaction between the DNA probe and the target sequence on viral RNA produces colour and electrochemical changes that could be visualised and detected by an electronic reader. This biosensor will be adapted as a strip/tube, to be used with a hand-held device that is user-friendly and cheap to manufacture. Prototype units of our biosensor will be tested against an appropriate number of clinical samples to estimate the performance of the device in sensitivity and predictivity. As an immediate effort for developing a diagnosis method during the current COVID19 outbreak, we aim to establish detection tool with a fast turnaround time of 30-45 minutes, from RNA extraction to result interpretation. Later, we plan to integrate the virus isolation and RNA extraction steps in the biosensor system through a microfluidic based-platform to upgrade its versatility as point-of-care testing device in resource-limited settings