Development of SARS-CoV-2 Molecular Virological Tools

Grant number: SS21026

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Key facts

  • Disease

    COVID-19
  • Start & end year

    2021
    2021
  • Known Financial Commitments (USD)

    $59,630
  • Funder

    Estonian Research Council
  • Principal Investigator

    Pirjo and Maria Cecilia Spuul and Sarmiento Guerin
  • Research Location

    Estonia
  • Lead Research Institution

    Tallinn University of Technology, Faculty of Science, Institute of Chemistry and Biotechnology
  • Research Priority Alignment

    N/A
  • Research Category

    Pathogen: natural history, transmission and diagnostics

  • Research Subcategory

    Pathogen morphology, shedding & natural history

  • Special Interest Tags

    N/A

  • Study Type

    Unspecified

  • Clinical Trial Details

    N/A

  • Broad Policy Alignment

    Pending

  • Age Group

    Not Applicable

  • Vulnerable Population

    Not applicable

  • Occupations of Interest

    Not applicable

Abstract

SARS-CoV-2 testing is performed in laboratories with BSL3 (biosafety level 3) safety levels, which are expensive and often not available. This has created a great need for solutions that allow SARS-CoV-2 materials to be used in lower safety laboratories. Based on this need, cooperation has been established with the virologists of the University of Tartu (Prof. Merits and Dr. Varjak). This project will have two outputs, the first of which are complementation systems (trans-replicases) based on SARS-CoV-2. Another output is the creation of leased lines that allow the virus replication machine to be packaged into particles that mimic functional virions. Such an approach would allow the impact of new mutations on viral replication to be investigated in the future. In addition, SARS-CoV-2 neutralizing antibodies and other anti-COVID-19 drug candidates can be tested. Thus, the development of these systems allows for close collaboration with hospitals to study the effects of antibodies isolated from patients.