Complete solution for molecular diagnosis of COVID 19 multiplex assay along with screening for other related respiratory diseases

We aim at optimizing and establish manufacturing of all the reagents required to perform real time PCR assay which includes sample collection, extraction and amplification. At present Huwel is manufacturing Enzymes and mixes. Although high quality enzymes and optimized mixes are main requirement for manufacturing of molecular assays, good quality primer and probes are required to achieve good sensitivity and stability. In India we realize that 95 of fluorescence probes are synthesised outside country and at the time of crisis it becomes bottle neck to procure sufficient quantity of probes. Moreover since most of the primers and probes are to imported, the supply to the researcher would take more than a month for simple probes. Next technology which is lacking in the country is nucleic acid extraction kits. In this case again we are dependent only on international suppliers. Molecular transport medium not only allows to transport intact Nucleic acid at room temperature but also inactivates the virus. For Countries like India, where containment facilities are not in abundance molecular sample transport medium becomes of utmost importance. Here we propose to develop molecular transport medium, establish manufacturing of different fluorescent probes, and development of silica coated magnetic beads which can be produced in bulk easily and automated easily for efficient extraction avoiding cross contamination. Detection Panel will include multiplex assay for COVID-19, as well as other related and symptomatically similar respiratory diseases.