(Theme 3) SARS-CoV-2 exit strategies
- Funded by UK Research and Innovation (UKRI)
- Total publications:0 publications
Grant number: C19-IUC-362
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Key facts
Disease
COVID-19Funder
UK Research and Innovation (UKRI)Principal Investigator
Jeremy CarltonResearch Location
United KingdomLead Research Institution
Francis Crick InstituteResearch Priority Alignment
N/A
Research Category
Pathogen: natural history, transmission and diagnostics
Research Subcategory
Pathogen morphology, shedding & natural history
Special Interest Tags
N/A
Study Type
Non-Clinical
Clinical Trial Details
N/A
Broad Policy Alignment
Pending
Age Group
Not Applicable
Vulnerable Population
Not applicable
Occupations of Interest
Not applicable
Abstract
The lifecycle of a virus requires release from infected cell through host cell membranes co-opting the host's own pathways for secretion. In the case of SARS-CoV-2 this budding is driven via the ESCRT pathway. We propose to perform a yeast 2-hybrid screen to generate tagged versions of SARS-CoV-2 structural proteins which will enable their visualisation during particle assembly and will allow us to use proteomic approaches to identify host proteins governing their membrane trafficking itineraries. We hope also to develop particle release assays for SARS-CoV-2. In doing so, we hope to understand more about late stages in the SARS-CoV-2 lifecycle.