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(Theme 3) SARS-CoV-2 exit strategies

  • Funded by UK Research and Innovation (UKRI)
  • Total publications:0 publications

Grant number: C19-IUC-362

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Key facts

  • Disease

    COVID-19

  • Start & end year

    N/A

  • Funder

    UK Research and Innovation (UKRI)

  • Principal Investigator

    Jeremy Carlton

  • Research Location

    United Kingdom

  • Lead Research Institution

    Francis Crick Institute

  • Research Priority Alignment

    N/A

  • Research Category

    Pathogen: natural history, transmission and diagnostics

  • Research Subcategory

    Pathogen morphology, shedding & natural history

  • Special Interest Tags

    N/A

  • Study Type

    Non-Clinical

  • Clinical Trial Details

    N/A

  • Broad Policy Alignment

    Pending

  • Age Group

    Not Applicable

  • Vulnerable Population

    Not applicable

  • Occupations of Interest

    Not applicable

Abstract

The lifecycle of a virus requires release from infected cell through host cell membranes co-opting the host's own pathways for secretion. In the case of SARS-CoV-2 this budding is driven via the ESCRT pathway. We propose to perform a yeast 2-hybrid screen to generate tagged versions of SARS-CoV-2 structural proteins which will enable their visualisation during particle assembly and will allow us to use proteomic approaches to identify host proteins governing their membrane trafficking itineraries. We hope also to develop particle release assays for SARS-CoV-2. In doing so, we hope to understand more about late stages in the SARS-CoV-2 lifecycle.