COVID-19 Variant Supplement - Development of a rapid point-of-care diagnostic test for COVID-19

A novel zoonotic coronavirus (SARS-CoV-2) has recently been identified in patients with an acute and potentially fatal respiratory disease (COVID-19). This virus is genetically similar to SARS and MERS coronaviruses. The outbreak started in the city of Wuhan (China) and then soon turned into a pandemic with over 60,000 clinical cases and at least 1,500 fatalities. Cases in healthcare workers and other close contacts indicate human-to-human transmission. Rapid, simple and specific point-of-care diagnostic tests are urgently needed for the quick isolation of those infected. To address the issue, we have assembled a team of specialists in chemistry, infectious diseases, and clinical diagnostics. We propose to develop rapid point-of-care tests based on aptamer-assisted graphene oxide (AptaGO) and paper enzyme-linked aptamer assays (p-ELAA) for SARS-CoV-2 and its surrogate (HCoV-229E), which is a Containment Level 2 (CL-2) pathogen. The aptamer-based sensors can rapidly (