COVID-19-popCell - Genetic and infectious factors underlying population variability in immune responses to SARS-CoV-2

About The popCell project aims to characterize the variation in immune response to SARS-CoV-2 of peripheral blood mononuclear cells (PBMCs) from > 200 adults, healthy volunteers of West European, Central African and East Asian origin, (EvoImmunoPop and SeroPrevHK-CoV- 19 cohorts). In this project, we have acquired single-cell transcriptional profiles (scRNA-seq) of >1M PBMCs following stimulation by SARS-CoV-2, Influenza A virus (IAV) or mock controls, in order to analyze the differences in immune response to SARS-CoV-2 among human populations worldwide. In addition, all donors were genotyped at >2.5 M sites using the Omni5 Illumina BeadChip, allowing to perform eQTL mapping and to define the role of genetics in these differences. These data will be integrated with chromatin accessibility data (scATAC-seq) and viral serologies (VIRscan) obtained in the basal state to identify regulatory regions that respond to environmental stimuli and contribute to the priming of immune responses to SARS-CoV-2. Any reanalysis of data published as part of the COVID-19 popCell project are listed below, together with the aim of the reanalysis and name of the person responsible for the data. List of data reuse: TBD