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Development of tools to sequence SARS-CoV-2 from wstewater samples

Grant number: LLTTI24238

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Key facts

  • Disease

    COVID-19

  • Start & end year

    2024
    2024

  • Known Financial Commitments (USD)

    $2,719,994.98

  • Funder

    Estonian Research Council

  • Principal Investigator

    Kisand Veljo

  • Research Location

    Estonia

  • Lead Research Institution

    University of Tartu

  • Research Priority Alignment

    N/A

  • Research Category

    Pathogen: natural history, transmission and diagnostics

  • Research Subcategory

    Pathogen genomics, mutations and adaptations

  • Special Interest Tags

    N/A

  • Study Type

    Non-Clinical

  • Clinical Trial Details

    N/A

  • Broad Policy Alignment

    Pending

  • Age Group

    Not Applicable

  • Vulnerable Population

    Not applicable

  • Occupations of Interest

    Not applicable

Abstract

The work aims to test the suitability of the Nanopore Oxford sequencing technology for the study of metagenomic material isolated from wastewater with the aim of identifying genomic variants of SARS-CoV-2 and to evaluate the possibilities of identifying other pathogens found in it. It is a preliminary study, the results of which depend on the possibilities and goals of further development work. A sequencing panel called the Ion AmpliSeq™ SARS-CoV-2 Research Panel is used for comparison.