Synthesis of fluorescent or bio-luminescent cDNA clone

Threat from RNA viruses normally spread among wild animals has always been considered as real but under-scored and neglected danger. Unfortunate events of this year highlight that this has been a serious mistake and we are ill-prepared for such kind of emergencies. Among other things we lack proper working tools and reliable animal models, in the absence of these researches cannot produce reliable data and counteract the problem. The project pre-dates COVID-19 pandemics but in essence, deals with the issues that have been proven to be problems when handling SARS-CoV-2 pandemics. The main goal is to develop tools for extremely dangerous alphavirus, Eastern Equine encephalitis virus, including animal model. The project does not involve work with infectious EEEV in Estonia thus there is no biological danger or threat for the environment.